Figure 1

Identification, selection and detection of the miR-200c expression in the CD44⁺ CD117⁺ CSCs. A. The CD117⁺CD44⁺CSCs isolated from the human EOC SKOV3 cell line were identified by FCM; B. The miR-200c expression differences between the non CD117⁺CD44⁺CSCs and the CD117⁺CD44⁺CSCs were detected by qRT-PCR; C. The top and bottom panels show the morphological structures of the CD44⁺CD117⁺CSCs with the stable miR-200c overexpression under a fluorescence microscope and a light microscope, respectively. The CD44⁺CD117⁺CSCs transduced with lentivirus-mock (left) or lentivirus miR-200c (right) in the stem cell culture medium; D. miR-200c expression differences among the CD44⁺CD117⁺CSCs transduced with lentivirus-mock, the lentivirus miR-200c and without lentivirus infection were detected by qRT-PCR. E. E-cadherin was detected by IHC assay in tumors of mice injected with the different cells. The labels ‘WT, mock and miR-200c’ denote the CD44⁺CD117⁺ CSCs, the CD44⁺CD117⁺ CSCs transduced with lentivirus-mock, and the lentivirus miR-200c, respectively. These labels are also used in Figures 2, 3, 4 below.