Figure 5

Down-regulation of ZEB1 inhibited the colony forming, cell migration and invasion in vitro . A-C. The representative images from the results of the plate clone forming assay, the wound healing assay, and the transwell invasive assay, respectively; D-F give the between-group statistical differences in the plate colony forming ratio, in the healing degree, and in the invasive cells, respectively. The labels ‘WT, scramble and shZEB1’ denote the CD44⁺CD117⁺CSCs, the CD44⁺CD117⁺CSCs stably transfected with scramble shRNA, and the CD44⁺CD117⁺CSCs stably transfected with shZEB1, respectively. *p < 0.05 and **p < 0.01. These labels are also used in Figure 6 below.