Figure 7

GPER-1 specific agonist G-1 induced expression of cyclin B1 and Cdc2 regulatory proteins, phosphorylation of histone 3 and cleavage of pro-caspase-3. SCOV-3 (A) and OVCAR-3 (B) ovarian cancer cells were incubated with medium (control) or 1 μM G-1 for indicated times and the protein expression of cyclin B1, Cdc2 and caspase-3 as well as the phosphorylation of histone 3 were investigated by western blot analysis. ß-actin was used as a loading control. Each experiment was repeated three times.