Relative mRNA expression (Y-axis) by qRT-PCR for Fsh-r1 and Fsh-r3 transcripts. Upper panel shows R1 transcript of FSH receptor mRNA expression in ( A ) initial scraped OSE and after ( B ) 3 hrs and ( C ) 15 hrs with (dark blue bars) and without (light blue bars) FSH treatment. Please note that R1 transcript levels are not affected much by FSH treatment. Lower panel shows R3 transcript of FSH receptor mRNA expression in ( D ) initial scraped OSE and after ( E ) 3 hrs ( F ) and 15 hrs with (red bars) and without (pink bars) FSH treatment. Note the increased expression (more than ten-fold) of R3 transcript after FSH treatment at 3 hrs followed by a reduction to basal levels at 15 hrs (please note a change in Y-axis scale to appreciate a difference between D-F). The relative expression levels of each transcript from three different experiments are represented individually (see Results section for more details).