| |
Sequence
|
Annealing temperature
|
Amplicon size
|
|---|
| |
FSH Receptor Transcripts
| | |
|
R1
|
CATTCACTGCCCACAACTTTCATC
|
60°C
|
84 bp
|
|
TGAGTGTGTAATTGGAACCATTGGT
|
|
R3
|
TCTCCACTGCTGCACTGTTGGGCT
|
55°C
|
382 bp
|
|
ATTCAAATACAGGAAATAGAGAAA
|
| |
Pluripotent Stem Cell Marker (VSELs)
| | |
|
Oct-4A
|
CAATTTGCCAAGCTCCTAAA
|
53°C
|
290 bp
|
|
TTGCCTCTCACTTGGTTCTC
|
|
Sox-2
|
TGATACGGTAGGAGCTTTGC
|
56°C
|
362 bp
|
|
CTTTTGCCCCTTTAGAGACC
|
| |
Differentiation Marker (OGSCs)
| | |
|
Oct - 4 (all isoforms)
|
GAGCCGAACCCTGAGGAGTCCC
|
66°C
|
225 bp
|
|
CAGCAGGGGCCGCAGCTTAC
|
| |
Housekeeping Gene
| | |
|
Gapdh
|
GCC CAG AAC ATC ATC CCT G
|
60°C
|
232 bp
|
|
GGT CCT CAG TGT AGC CTA G
|
-
Oct-4A is a true marker for pluripotent stem cells
[33]. Oct-4 primers amplify both Oct-4A and other isoforms including Oct-4B. Oct-4A reflects VSELs whereas several fold increase in Oct-4 reflects increase in the number of OGSCs suggesting differentiation.
