Figure 1

WDR62 expression in ovarian cancer cell lines. (A) Whole cell lysates from multiple ovarian cancer cell lines were subjected to Western blot using primary anti-WDR62 antibody. (B) Cells in culture was exposed to anti-centrosome antibodies. After secondary antibody-FITC (green), cells were exposed to 4’, 6’-diamidino-2-phenylindole to stain nuclei blue and examined by fluorescent microscopy. White arrows indicated centrosomes. (C) After fluorescent staining with anti-centrosome antibodies, the number of cells with more than two centrosomes was counted (at least 400 cells counted) and compared between cell lines.