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Figure 3 | Journal of Ovarian Research

Figure 3

From: Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

Figure 3

GPER mRNA assayed and normalized to IPO8, RPL4, GADPH, and HPRT1 mRNA. Ovarian tumours were sub-grouped according to the histological malignant potential as benign (BE, n = 9), borderline (BO, n = 11) and malignant (MA, n = 22). Normalization to IPO8 and RPL4 showed no significant variation of the GPER mRNA content between BE, BO and MA tumours (A, B). In contrast, GPER mRNA was higher in BE/BO compared to MA when normalized to GADPH (p = 0.002) or HPRT1 (p = 0.008) (C, D).

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