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Table 2 Reference genes, target genes and assays used

From: Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

Gene symbol Gene name(synonyms) Function NCBI Gene reference Assay ID
ABL1 C-abl oncogene 1, non-receptor tyrosine kinase Cell differentiation, division, adhesion and stress response. NM_005157.3, NM_007313.2 Hs00245445_m1
ACTB Actin, beta Cell motility, structure, integrity NM_001101.3 Hs99999903_m1
CDKN1A Cyclin-dependent kinase inhibitor 1A (p21, Cip1) Regulation of cell cycle progression at G1. NM_004064.3 Hs00355782_m1
GADPH Glyceraldehyde-3-phosphate dehydrogenase Catalysation of an important energy-yielding step in carbohydrate metabolism. NM_002046.3 Hs99999905_m1
GUSB Glucuronidase, beta Degradation of glycosaminoglycans NM_000181.2 Hs99999908_m1
HPRT1 Hypoxanthine phosphoribosyl transferase 1 Generation of purine nucleotides through the purine salvage pathway. NM_000194.2 Hs99999909_m1
HSP90AB1 Heat shock protein 90 Protein folding, response to stress. NM_007355 Hs.PT.49a.20846338
IPO8 Importin 8 Nuclear transport. NM_001190995.1 NM_006390.3 Hs00183533_m1
PPIA Peptidylprolyl isomerase A (cyclophilin A) Protein folding, ligand for Cyclosporin A. NM_021130.3 Hs99999904_m1 Hs.PT.39a.22214851
RPL30 Ribosomal protein L30 Component of 60S subunit. Catalysation of protein synthesis. NM_000989.2 Hs00265497_m1
RPL4 Ribosomal protein L4 Component of 60S subunit. NM_000968 Hs.PT.49a.20266660
RPLPO Ribosomal protein, large, PO Component of 60S subunit. NM_053275.3, NM_001002.3 Hs99999902_m1
TBP TATA box binding protein Initiation of transcription of RNA polymerases. M34960.1 M55654.1 Hs99999910_m1
GPER G protein-coupled estrogen receptor Rapid estrogen signalling. NM_001505.2 Hs00173506_m1
uPAR Urokinase plasminogen activator receptor Cell invasion, migration, signalling via ERK1/2. NM_001005376.2 NM_001005377.2 NM_002659.3 Hs00182181_m1