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Table 2 Reference genes, target genes and assays used

From: Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

Gene symbol

Gene name(synonyms)

Function

NCBI Gene reference

Assay ID

ABL1

C-abl oncogene 1, non-receptor tyrosine kinase

Cell differentiation, division, adhesion and stress response.

NM_005157.3, NM_007313.2

Hs00245445_m1

ACTB

Actin, beta

Cell motility, structure, integrity

NM_001101.3

Hs99999903_m1

CDKN1A

Cyclin-dependent kinase inhibitor 1A (p21, Cip1)

Regulation of cell cycle progression at G1.

NM_004064.3

Hs00355782_m1

GADPH

Glyceraldehyde-3-phosphate dehydrogenase

Catalysation of an important energy-yielding step in carbohydrate metabolism.

NM_002046.3

Hs99999905_m1

GUSB

Glucuronidase, beta

Degradation of glycosaminoglycans

NM_000181.2

Hs99999908_m1

HPRT1

Hypoxanthine phosphoribosyl transferase 1

Generation of purine nucleotides through the purine salvage pathway.

NM_000194.2

Hs99999909_m1

HSP90AB1

Heat shock protein 90

Protein folding, response to stress.

NM_007355

Hs.PT.49a.20846338

IPO8

Importin 8

Nuclear transport.

NM_001190995.1 NM_006390.3

Hs00183533_m1

PPIA

Peptidylprolyl isomerase A (cyclophilin A)

Protein folding, ligand for Cyclosporin A.

NM_021130.3

Hs99999904_m1 Hs.PT.39a.22214851

RPL30

Ribosomal protein L30

Component of 60S subunit. Catalysation of protein synthesis.

NM_000989.2

Hs00265497_m1

RPL4

Ribosomal protein L4

Component of 60S subunit.

NM_000968

Hs.PT.49a.20266660

RPLPO

Ribosomal protein, large, PO

Component of 60S subunit.

NM_053275.3, NM_001002.3

Hs99999902_m1

TBP

TATA box binding protein

Initiation of transcription of RNA polymerases.

M34960.1 M55654.1

Hs99999910_m1

GPER

G protein-coupled estrogen receptor

Rapid estrogen signalling.

NM_001505.2

Hs00173506_m1

uPAR

Urokinase plasminogen activator receptor

Cell invasion, migration, signalling via ERK1/2.

NM_001005376.2 NM_001005377.2 NM_002659.3

Hs00182181_m1