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Table 3 Descriptive and correlation analysis of the candidate RGs obtained by BestKeeper

From: Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

  ABL1 ACTB CDKN1A GADPH GUSB HPRT1 HSP90 IPO8 PPIA RPL30 RPL4 RPLPO TBP
n 41 42 42 41 42 41 42 42 42 42 42 42 42
gM [ C t ] 28.05 23.73 28.54 25.39 31.20 29.02 26.81 29.10 22.12 28.78 25.88 24.86 28.70
aM [ C t ] 28.07 23.75 28.57 25.42 31.23 29.04 26.84 29.11 22.15 28.81 25.90 24.88 28.71
min [ C t ] 25.90 21.80 26.43 23.02 27.75 26.63 24.30 27.48 19.91 26.34 23.79 22.91 27.28
max [ C t ] 30.39 25.87 31.23 27.80 34.06 31.91 29.55 30.64 24.53 31.06 27.98 26.66 31.55
SD C t ] 0.87 0.73 1.05 1.05 0.99 0.91 0.86 0.65 0.82 1.09 0.77 0.81 0.75
CV [% C t ] 3.10 3,07 3.69 4.11 3.17 3.13 3.19 2.22 3.71 3.78 2.98 3.27 2.62
min [ x - fold ] −3.62 −3.36 −4.00 −4.33 −10.12 −4.17 −5.66 −2.76 −4.17 −4.53 −3.79 −3.37 −2.62
max [ x - fold ] 4.04 3.85 5.85 4.41 6.78 5.64 6.62 2.61 4.73 4.11 3.82 3.06 6.15
SD x - fold ] 1.68 1.55 1.88 1.87 1.81 1.72 1.67 1.47 1.63 1.96 1.61 1.66 1.59
  1. Geometric mean of Ct (gM [C t ]), arithmetic mean (aM [C t ]), minimum and maximum values of Ct (min [C t ], max [C t ]), standard deviation of Ct (SDC t ]), coefficient of variance expressed as a percentage on the Ct level (CV [% C t ]), extreme values of expression levels expressed as an absolute x-fold over- or under- regulation coefficient (min [x-fold], max [x-fold]), and standard deviation of the absolute regulation coefficients (SDx-fold]).