Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

Table 3 Descriptive and correlation analysis of the candidate RGs obtained by BestKeeper

	ABL1	ACTB	CDKN1A	GADPH	GUSB	HPRT1	HSP90	IPO8	PPIA	RPL30	RPL4	RPLPO	TBP
n	41	42	42	41	42	41	42	42	42	42	42	42	42
gM [ C _t ]	28.05	23.73	28.54	25.39	31.20	29.02	26.81	29.10	22.12	28.78	25.88	24.86	28.70
aM [ C _t ]	28.07	23.75	28.57	25.42	31.23	29.04	26.84	29.11	22.15	28.81	25.90	24.88	28.71
min [ C _t ]	25.90	21.80	26.43	23.02	27.75	26.63	24.30	27.48	19.91	26.34	23.79	22.91	27.28
max [ C _t ]	30.39	25.87	31.23	27.80	34.06	31.91	29.55	30.64	24.53	31.06	27.98	26.66	31.55
SD [± C _t ]	0.87	0.73	1.05	1.05	0.99	0.91	0.86	0.65	0.82	1.09	0.77	0.81	0.75
CV [% C _t ]	3.10	3,07	3.69	4.11	3.17	3.13	3.19	2.22	3.71	3.78	2.98	3.27	2.62
min [ x - fold ]	−3.62	−3.36	−4.00	−4.33	−10.12	−4.17	−5.66	−2.76	−4.17	−4.53	−3.79	−3.37	−2.62
max [ x - fold ]	4.04	3.85	5.85	4.41	6.78	5.64	6.62	2.61	4.73	4.11	3.82	3.06	6.15
SD [± x - fold ]	1.68	1.55	1.88	1.87	1.81	1.72	1.67	1.47	1.63	1.96	1.61	1.66	1.59

Geometric mean of C_t (gM [C _t]), arithmetic mean (aM [C _t]), minimum and maximum values of C_t (min [C _t], max [C _t]), standard deviation of C_t (SD [± C _t]), coefficient of variance expressed as a percentage on the C_t level (CV [% C _t]), extreme values of expression levels expressed as an absolute x-fold over- or under- regulation coefficient (min [x-fold], max [x-fold]), and standard deviation of the absolute regulation coefficients (SD [± x-fold]).

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