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Table 5 NormFinder ranking of 13 candidate RGs and combinations of the two best in group-wise comparison

From: Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

Gene name

BE × BO × MA

BE+BO × MA

BE × BO+MA

BE × MA

Ser × Muc(BE+BO)

Ser × End (MA)

ALB1

13

13

13

13

9

9

ACTB

2

4

6

5

7

4

CDKN1A

12

12

8

8

12

11

GADPH

8

9

11

11

10

10

GUSB

7

7

12

12

11

12

HPRT1

10

8

7

7

8

5

HSP90

6

11

5

6

2

8

IPO8

5

3

2

1

1

2

PPIA

9

10

9

9

4

3

RPL30

11

6

10

10

13

13

RPL4

1

1

3

2

3

7

RPLPO

4

2

4

3

6

6

TBP

3

5

1

4

5

1

Best combination

RPL4/ACTB

RPL4/RPLPO

IPO8/TBP

IPO8/RPL4

IPO8/HSP90

IPO8/TBP

M-value

0.104

0.088

0.060

0.079

0.060

0.073

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Journal of Ovarian Research

ISSN: 1757-2215

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