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Table 6 Expression stability of the candidate RGs analysed by equivalence test

From: Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours – IPO8 and RPL4 are reliable reference genes

  BE × BO+MA BE+BO × MA BE × MA Ser × Muc (BE+BO) Ser × End(MA) Total passes 2-fold/3-fold
ABL1 0 /1 0 /1 0 /1 1 /1 0 /1 1 /5
ACTB* 0 /1 0 /1 0 /1 1 /1 0 /1 1 /5
CDKN1A 0 /1 1 /1 0 /1 0 /1 0 /1 1 /5
GADPH 0 /0 0 /0 0 /0 0 /1 0 /1 0 /2
GUSB 0 /1 0 /1 1 /1 1 /1 0 /1 2 /5
HPRT1 0 /1 0 /0 0 /0 0 /1 0 /1 0 /3
HSP90 0 /1 0 /0 0 /0 0 /1 0 /1 0 /3
IPO8* 1 /1 1 /1 1 /1 1 /1 1 /1 5 /5
PPIA 0 /1 0 /0 0 /0 1 /1 0 /1 1 /3
RPL30 1 /1 0 /1 0 /1 0 /1 1 /1 2 /5
RPL4* 1 /1 0 /1 0 /1 0 /1 1 /1 2 /5
RPLPO* 0 /1 0 /1 0 /1 0 /1 1 /1 1 /5
TBP* 1 /1 0 /1 0 /1 0 /1 1 /1 2 /5
  1. The expression within (1) or outside (0) 2-fold/3-fold expression change cut-off and the total number of meeting the cut-off criteria in the five subgroups.
  2. * Genes best-ranked by GeNorm, NormFinder and BestKeeper.