Figure 4
From: Tracking NF-κB activity in tumor cells during ovarian cancer progression in a syngeneic mouse model
Analysis of fluid collected from the peritoneal cavity. (A) Representative images of cytospin analyses of peritoneal lavage fluid/ascites collected from mice injected with tumor cells (Inject) or mock-PBS-injected mice (Non). Tumor cell clumps are shown (yellow arrow). (B) Overall number of mononuclear cells collected increased in mice with tumors and with duration of progression. (C) Expression of the epithelial marker, pan-cytokeratin (CK; red), and the mature macrophage marker, F4/80 (green), in ascites fluid on cytospin slides. Percentage of CK-positive cells is shown in (D). Values are mean + SD of counts from 200 cells from each of 3 mice per group. * p < 0.01 relative to non-inject, Mann-Whitney test.