Figure 14

Zygote scoring system of De Placido et al. [17]. The authors combined five previous scoring systems [10, 11, 50–52] and considered 3 main parameters: (i) the position of PN in relation to the cytoplasm (A-E); (ii) the morphology and orientation of nucleoli (F-L); (iii) the presence of a dense area of the cytoplasm aggregate around the PN (cytoplasmic flare) (M-Q). Zygotes showing two opposed PN with equal size in centre of the cytoplasm, equal number of juxtaposed nucleoli and the cytoplasmic flare were considered as top quality zygotes.