Figure 2
Zygote scoring system of Scott and Smith [50]. In this classification, if PN are close or aligned they are assigned a score 5 (A). If they are well separated or unequal in size they are classified as score 1 (B). Nucleoli aligned in a row at the PN junction are scored as 5 (C), beginning to align as 4 (D), scattered as 3 (E). The cytoplasm is scored as follow: heterogeneous in appearance with a clear halo around the edges, occasionally with a clear area in the centre around the PN and darkened ring/halo in the middle, scored 5 (F), whereas zygotes with a clear homogeneous cytoplasm or a pitted and/or darkened cytoplasm scored 6 (G).