Figure 3

Zygote scoring system of Tesarik and Greco [51]. pattern 1 includes zygotes with big difference (>3) in the number of NPBs in both PN (A), pattern 2 includes zygotes showing a small number (<7) of NPBs without polarization in at least one PN (B), pattern 3 includes zygotes with a large number (>7) of NPBs with polarization in at least one PN (C), pattern 4 includes zygotes characterized by a very small number (<3) of NPBs in at least one PN (D) and pattern 5 includes zygotes showing polarized distribution of NPBs in one PN and non-polarized in the other. These 5 patterns are considered as “abnormal”; zygotes not included in pattern 1–5 are classified as pattern 0, and are considered “normal” (F and G).