Ovaries of WT mice. Ovaries of 2.5-year-old WT mice fed AL (panels A and C) and WT mice on CR (panels B and D). In WT mice on an AL diet (panels A and C), a cyst (asterisk) is visible that shows a positive reaction for apoptosis in a few granulosa cells (black arrow) inside the cyst and in a few stromal cells (yellow arrow) surrounding the cyst (panel C). In WT mice on caloric restriction (panels B and D), single antral follicles (AF), single Graffian follicle, enlarged interstitial tissue (IT), and macrophages (M) are visible. A positive apoptotic reaction is detectable in a few granulosa cells in apical parts (cross section) of Graffian follicle (black arrow, panels B and D). Magnification: panels A and B x40; panels C and D x400. Insert in panel D represents OSE with absence of apoptotic cells. Magnification: x800.