Ovaries of GHR-KO mice. Ovaries of 2.5-year-old GHR-KO mice fed AL (panels A, C, and E) and 2.5- year-old GHR-KO mice on CR (panels B, D, and F). In both groups, primordial (PRF), primary (PF), preantral (PAF), antral (AF) and Graafian (GF) follicles are visible. A positive reaction for apoptosis was detectable in granulosa cells (arrow) and in ovarian surface epithelium (arrowhead) in both groups. Interstitial cells (IT), macrophages (M). Magnification: panels A and B x40; panels C and D x200; panels E and F x400. Insert in panel E represents primordial follicles located under OSE. Magnification: x800. Insert in panel F represents OSE with positive apoptotic reaction. Magnification: x800.