Figure 2

In vitro mouse preantral follicle culture system and the effects of FSH on follicular development. Examples of follicular development treated with or without follicle stimulating hormone (FSH) (100 mIU/mL) are shown. The majority of follicles not treated with FSH (control) grew in a multilayered pattern and did not survive. On the other hand, follicles cultured with FSH (100 mIU/mL) grew larger and formed antral-like cavities at 10 to 12 days of culture. The cumulus oocyte complex was visibly extruded from the antral-like cavity after 16 h of the administration of 5 IU/mL of hCG (inset). Bar = 100 μm.