Figure 2
Effect of various citrate (CT) concentrations (5, 10, 20 mM) in IGROV1-R10 cells after 24 and 72 H exposure. (A) The morphological features of cell layers were observed by photon microscopy. (B) DNA content was determined at 24 H or 72 H by flow cytometry after propidium iodide staining (for each condition, percentages of cells in the different phases of the cell cycle are indicated). Protein expression levels of PARP cleavage and caspase 3 cleavage and Mcl-1 and Bcl-xL on SKVO3 cells (C) and on IGROV1-R10 cells (D) by western blot analysis in response to citrate at 6 and 24 H. Mcl-1 mRNA expression in SKOV3 cells (E) and IGROV1-R10 cells (F) treated with citrate for 6 H or 24 H was assessed using real-time quantitative reverse transcription PCR. GAPDH was used as a housekeeping reference gene for normalisation. Each relative mRNA expression level was calculated in comparison to the control cell expression level.