Figure 3

Effect of siXL1 in response to citrate in the SKOV3 cell line. Cells were transfected with 20 nM siXL1 24 H before exposure to 10 mM of citrate and cultured for an additional 48 H (i.e. 72 H post transfection). Evolution of viable cells up to 72 H (A) in SKOV3. Results are expressed as a percentage of viable cells compared to the 100% control cells. Results are expressed as the mean values of two independent experiments. Analysis of variance was used to determine significance. **: p < 0.05; ***: p < 0.001. (B) Cell cycle repartition was studied by flow cytometry after propidium iodide staining at 72 H after transfection in SKOV3 cell lines. (C) The nuclear morphology of SKOV3 cells was analysed by DAPI staining at 72 H. Bars 20 μm.