Figure 3

Multi- and pair-comparison statistical tests suggest that a combination of stage IIIC and grade G2 is the diagnosis associated with the highest levels of LSD1 mRNA overexpression in our cohort. Top panel: multiple-comparison analysis of normal tissue (N) and tumors subclassified as stage I or II and grade G2 (I-II/G2) or as stage I or II and grade G3 (I-II/G3); as stage III excluding IIIC and grade G2 (III-noIIIC/G2) or as stage III excluding IIIC and grade G3 (III-noIIIC/G3); and as stage IIIC and grade G2 (III/G2) or as stage IIIC and grade G3 (III/G3). Bottom panels: pair-comparison analyses in normal tissue (N), and stage IIIC and grade G2 (IIIC/G2) tumors (first panel); stage IIIC tumors, and stage IIIC and grade G2 (IIIC/G2) tumors (second panel); grade G2 tumors, and stage IIIC and grade G2 (IIIC/G2) tumors (third panel); stage IIIC and grade G3 (IIIC/G3) tumors, and stage IIIC and grade G2 (IIIC/G2) tumors (fourth panel); normal tissue (N), and stage IIIC and grade G3 (IIIC/G3) tumors (fifth panel); stage I or II and grade G3 (I-II/G3) tumors, and stage IIIC and grade G3 (IIIC/G3) tumors (sixth panel); and stage I or II and grade G2 (I-II/G2) tumors, and stage I or II and grade G3 (I-II/G3) tumors (seventh panel). We applied the Mann–Whitney test in pair-comparison analyses, and the Kruskal-Wallis (non-parametric ANOVA) test followed by post hoc Dunn’s analysis in multiple-comparison analyses (number of comparisons = 21). P-values are shown on top of each panel when significant. Number of specimens in each group is shown at the bottom of each panel. All histological subtypes were analyzed, excluding mucinous. Whiskers in box plots represent 5–95 percentile values, and horizontal lines within boxes represent median values. P-value < 0.05 (*), p-value < 0.01 (**), p-value < 0.001 (***), p-value < 0.0001 (****).