Table 3 Levels of CA-125, HE4, and 14-3-3 Z protein determined by ELISA and ECLIA methods
| Â | Â | Â | ELISA | Â | Â | ECLIA | Â | ELISA |
|---|---|---|---|---|---|---|---|---|
Patient no. | Chemo | CA-125 | HE4 | ROMA | CA-125 | HE4 | ROMA | 14-3-3 Z |
| Â | Cyle no. | (U/mL) | (p/M) | % | (U/mL) | (p/M) | % | (ng/mL) |
1 | 1 | 23.38 | 95.87 | 26.19 | 105.70 | 79.63 | 46.90 | 38.28 |
| Â | 2 | 18.09 | 65.94 | 16.60 | 76.82 | 30.69 | 20.57 | 37.95 |
| Â | 3 | 7.78 | 64.47 | 9.49 | 73.55 | 15.77 | 11.20 | 18.78 |
| Â | 4 | NA | NA | NA | NA | NA | NA | NA |
| Â | 5 | 10.71 | 67.85 | 12.26 | 77.76 | 8.58 | 6.5 | 32.01 |
| Â | 6 | 7.90 | 62.81 | 9.35 | 75.21 | 9 | 6.60 | 39.82 |
2 | 1 | 26.93 | 334.49 | 58.99 | 353.50 | 176.10 | 83 | 0 |
| Â | 2 | 24.28 | 102.01 | 28 | 116.10 | 106.20 | 56 | 0 |
| Â | 3 | 19.31 | 66.42 | 17.37 | 79.16 | 29.24 | 20.10 | 0 |
| Â | 4 | 10.16 | 87.74 | 14.97 | 88.92 | 18.22 | 14.30 | 0 |
| Â | 5 | 3.33 | 81.38 | 6.7 | 88.28 | 15.06 | 12 | 0 |
| Â | 6 | 0.08 | 85.94 | 0.49 | 87.33 | 13.11 | 10.50 | 0 |
3 | 1 | 1.45 | 43.73 | 2 | 83.1 | 52.97 | 32.6 | 0 |
| Â | 2 | 0 | 49.24 | 0 | 67.49 | 28.04 | 17.70 | 0 |
| Â | 3 | 3.47 | 52.41 | 4.48 | 69.24 | 23.49 | 15.4 | 0 |
| Â | 4 | NA | NA | NA | NA | NA | NA | NA |
| Â | 5 | 0 | 44.09 | 0 | 52.38 | 17.25 | 9.7 | 0 |
| Â | 6 | 9.12 | 52.37 | 8.68 | 62.76 | 24.63 | 15.10 | 0 |
4 | 1 | 0 | 48.51 | 0 | 55.06 | 6.78 | 4 | 1.34 |
| Â | 2 | 0 | 55.9 | 0 | 58.43 | 6.91 | 4.30 | 1.42 |
| Â | 3 | 3.63 | 47.48 | 4.18 | 54.72 | 6.71 | 4 | 0.39 |
| Â | 4 | 2.55 | 45.86 | 3.15 | 53.12 | 6.08 | 3.50 | 0.32 |
| Â | 5 | 4.35 | 51.67 | 5.16 | 58.04 | 6.59 | 4.10 | 0.26 |
| Â | 6 | NA | NA | NA | NA | NA | NA | NA |
5 | 1 | 9.23 | 101.40 | 15.93 | 133.4 | 14.60 | 15.20 | 0 |
| Â | 2 | 12.40 | 93.07 | 17.77 | 107.40 | 8.69 | 8.20 | 0 |
| Â | 3 | 2.78 | 85.49 | 6.21 | 103.30 | 7.08 | 6.50 | 0 |
| Â | 4 | 14.24 | 69.76 | 15.03 | 75.26 | 6.66 | 4.90 | 0 |
| Â | 5 | 9.10 | 75.68 | 12.20 | 80.91 | 7.95 | 6.20 | 0 |
| Â | 6 | 9.73 | 74.47 | 12.55 | 80.21 | 7.89 | 6.10 | 0 |
6 | 1 | 153.04 | 904 | 93.53 | 1500 | 701.5 | 98.30 | 0 |
| Â | 2 | 445.65 | 904 | 96.94 | 1218 | 644.20 | 97.90 | 0 |
| Â | 3 | 368.55 | 714.78 | 95.57 | 843.10 | 394.10 | 95.50 | 0 |
| Â | 4 | 249.51 | 541.89 | 92.41 | 540.10 | 243.90 | 90.30 | 0 |
| Â | 5 | 95.43 | 259.90 | 73.68 | 227.30 | 84.13 | 65.40 | 0 |
| Â | 6 | 39.94 | 167.57 | 48.43 | 142 | 46.16 | 38.30 | 0 |
7 | 1 | 77.86 | 118.41 | 51.49 | 111.90 | 68.66 | 44.10 | 4.36 |
| Â | 2 | 17.01 | 122.23 | 26.50 | 124.90 | 22.64 | 22.64 | 15.02 |
| Â | 3 | 9.25 | 120.03 | 18.50 | 127 | 18.20 | 17.80 | 8.28 |
| Â | 4 | 8 | 107.76 | 15.47 | 111.10 | 16.34 | 15 | 6.38 |
| Â | 5 | 1.07 | 53.14 | 1.98 | 132.50 | 15.39 | 15.80 | 13.13 |
| Â | 6 | 9.03 | 202.84 | 27.82 | 186.80 | 26.99 | 30.30 | 5.74 |
8 | 1 | 475.23 | 259.74 | 90.07 | 248.50 | 4810 | 99.2 | 0.04 |
| Â | 2 | 500 | 90.83 | 75.92 | 80.07 | 1265 | 92.7 | 0 |
| Â | 3 | 189.18 | 67.99 | 53.34 | 70.60 | 642.10 | 85.20 | 0 |
| Â | 4 | 500 | 904 | 97.18 | 1500 | 5000 | 99.8 | 0 |
| Â | 5 | 172.63 | 57.11 | 47.22 | 57.21 | 254.10 | 65.30 | 0 |
| Â | 6 | 402.03 | 62.74 | 64.64 | 57.66 | 256.20 | 65.60 | 0 |
9 | 1 | 21.16 | 904 | 77.35 | 1500 | 185.40 | 93.70 | 2.46 |
| Â | 2 | 29.53 | 170.69 | 43.41 | 176.10 | 61.61 | 49.50 | 1.61 |
| Â | 3 | 4.97 | 97.08 | 10.38 | 103.70 | 21.29 | 18.10 | 1.32 |
| Â | 4 | 8.13 | 96.13 | 14.09 | 102.70 | 15.37 | 13.50 | 0 |
| Â | 5 | 5.90 | 105.57 | 12.56 | 108.90 | 13.93 | 12.80 | 0.13 |
| Â | 6 | 8.57 | 84.25 | 12.96 | 87.24 | 15.70 | 12.40 | 0.06 |
10 | 1 | 500 | 904 | 97.18 | 1500 | 5000 | 99.8 | 0 |
| Â | 2 | 189.91 | 62.04 | 51.08 | 60.85 | 243.10 | 65.30 | 0 |
| Â | 3 | 500 | 441.44 | 94.22 | 514.20 | 4789 | 99.5 | 0 |
| Â | 4 | 500 | 149.23 | 84.07 | 155.70 | 833.50 | 93.10 | 0 |
| Â | 5 | 84 | 127.48 | 54.97 | 121.70 | 197.90 | 71.60 | 0 |
| Â | 6 | 29.43 | 90.79 | 28.37 | 92.78 | 54.27 | 35 | 0 |
11 | 1 | 7.85 | 904 | 62.12 | 1500 | 117.70 | 90.20 | 0 |
| Â | 2 | 9.58 | 201.95 | 28.59 | 200.90 | 36.28 | 38.40 | 0 |
| Â | 3 | 0.93 | 131.04 | 4.42 | 125.90 | 18.88 | 18.30 | 0 |
| Â | 4 | 3.75 | 105.53 | 9.34 | 104.40 | 13.74 | 12.30 | 0 |
| Â | 5 | 2.65 | 107.64 | 7.54 | 107.80 | 13.41 | 12.30 | 0 |
| Â | 6 | NA | NA | NA | NA | NA | NA | NA |
12 | 1 | 126.02 | 397.04 | 84.2 | 404.30 | 1772 | 98.30 | 0.38 |
| Â | 2 | 500 | 365.72 | 93.07 | 323.20 | 1517 | 97.70 | 0 |
| Â | 3 | 192.79 | 273.67 | 83.2 | 258.90 | 761.40 | 94.70 | 0.09 |
| Â | 4 | 178.79 | 223.37 | 79.09 | 192.20 | 360.10 | 86.80 | 0 |
| Â | 5 | 121.86 | 168.58 | 68.17 | 169 | 265.70 | 81.30 | 0 |
| Â | 6 | NA | NA | NA | NA | NA | NA | NA |
13 | 1 | 500 | 904 | 97.18 | 1500 | 5000 | 99.8 | 0 |
| Â | 2 | NA | NA | NA | NA | NA | NA | NA |
| Â | 3 | 500 | 904 | 97.18 | 1500 | 5000 | 99.8 | 0 |
| Â | 4 | 500 | 904 | 97.18 | 853.90 | 3241 | 99.50 | 0 |
| Â | 5 | 120.65 | 355.03 | 82.16 | 349.30 | 171.90 | 82.5 | 0 |
| Â | 6 | NA | NA | NA | NA | NA | NA | NA |