Figure 1From: Targeted gene silencing using a follicle-stimulating hormone peptide-conjugated nanoparticle system improves its specificity and efficacy in ovarian clear cell carcinoma in vitro Expression of FSHR and gro-α in human ovarian cancer cell lines SKOV-3 and ES-2. (A) Detection of FSHR and gro-α expression by immunocytochemistry. 1, 2 and 3, ES-2 cells stained with FSHR antibody, gro-α antibody and control IgG, respectively; 4, 5 and 6, SKOV-3 cells stained with FSHR antibody, gro-α antibody and control IgG, respectively. Bar, 20 μm. (B) Detection of gro-α mRNA expression by RT-PCR. M, DNA marker; lanes 1 and 2, gro-α mRNA expression in ES-2 and SKOV-3 cells, respectively (226 bp); lanes 3 and 4, ACTB expression in ES-2 and SKOV-3 cells, respectively (208 bp).Back to article page