Figure 5

The proliferation, migration and invasion of ES-2 cells after treatment with gro-α siRNA-loaded NPs. (A) ES-2 cell proliferation as determined by CCK-8 assay. Cells were incubated with serum-free medium containing G-NP or FSH33-G-NP for 4 h. At 24 h, 48 h, 72 h and 96 h, CCK-8 solution was added, and OD values at 450 nm were measured. (B) Determination of ES-2 cell migration by scratch assay. The cell monolayer was scraped 72 h after treatment. Cells were then photographed under light microscopy at 12 h and 36 h after scraping. (C) Determination of ES-2 cell invasion by transwell migration assay. 1, untreated cells; 2, cells treated with G-NP; 3, cells treated with FSH33-G-NP. Bar, 20 μm. Cells were harvested at 24 h after treatment and seeded into the upper chambers of transwell plates pre-coated with matrigel. After 24 h, cells were fixed, stained and examined by light microscopy. (D) Determination of cell migration distances by scratch assay at 36 h. (E) Determination of migrated cell counts by transwell migration assay.