Figure 2

Involvement of integrin/FAK signalling in OPG-mediated protection from TRAIL. (A) CaOV3 cells were incubated with αvβ3 and αvβ5 integrin blocking antibodies (5 μg/ml) for 1 h. Cells were washed and incubated with OPG (25 ng/ml). After 1 h, cells were washed and TRAIL (50 ng/ml) was added for 24 h and apoptosis was assessed. Apoptosis is expressed as fold increase relative to control (untreated) cells with the mean of triplicates from three independent experiments ± SD. (B) CaOV3 cells were incubated with 25 ng/ml OPG for 1 h. Cells were lysed and subjected to immunoblotting to detect total and phosphorylated FAK. Densitometric quantification of phosphorylated FAK from three separate experiments normalized to total FAK was performed.