Sequence variation among members of the miR-200 microRNA family is correlated with variation in the ability to induce hallmarks of mesenchymal-epithelial transition in ovarian cancer cells

Table 1 Prediction of miR-200 binding sites on FN1, ZEB1, ZEB2, KRT7, KRT8 and KRT18 mRNAs

miRNA	miR-200b			miR-200c			miR-429			miR-200a			miR-141
Prediction algorithm	†A	¥B	ǂC	A	B	C	A	B	C	A	B	C	A	B	C
FN1	2	2	1	2	2	1	2	2	1	0	0	0	0	0	0
ZEB1	5	6	5	5	6	5	5	6	5	3	3	3	3	3	3
ZEB2	6	5	6	6	5	6	6	5	6	3	4	2	3	4	2
KRT7	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
KRT8	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
KRT18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

Only those genes predicted to be targeted by miR-200 family members were observed to be downregulated in cells in which they were ectopically overexpressed.
†A: miRanda (mirSVR ≤ -0.2), ¥B: miRDB, ǂC: TargetScan (conserved miRNA sites).

ISSN: 1757-2215