Total, M1, and M2 TAMs immunostaining in ovarian cancer tissues. (A) Immunohistochemical staining of CD68+ TAMs in various ovarian cancer histotypes. The arrowheads indicate CD68+ TAMs. The scale bars are 100 μm (upper row) and 25 μm (lower row). (B) Immunofluorescent staining of CD68+ TAMs expressing a single cellular differentiation biomarker (CD68+iNOS+/CD68+HLA-DR+ for M1 and CD68+CD163+/CD68+VEGF+ for M2) in ovarian cancer tissue. The arrowheads indicate M1 or M2 TAMs. (C) Immunofluorescent staining of TAMs co-expressing the M1 and M2 biomarkers in ovarian cancer tissue and the co-immunostaining percentages for different biomarker combinations in total TAMs. The arrowhead indicates TAMs that were co-immunostained with M1 and M2 biomarkers.