Effect of simvastatin (1 μM) and resveratrol (3-10 μM) on mRNA expression of Star (A), Cyp11a1 (B), Hsd3b1 (C) and Cyp17a1 (D). Theca-interstitial cells were cultured in chemically defined media supplemented with LH (5 ng/ml) for 48 h in the absence (control) or in the presence of simvastatin and/or resveratrol. Total RNA was isolated, and mRNA expression was determined using quantitative real-time PCR reactions and normalized to Hprt mRNA levels. Results are presented as a percentage of control. Each bar represents mean ± SEM from three independent experiments (each with N = 4). Means with no superscripts in common are significantly different (P <0.05).