Figure 3

Inhibitory effect of IT +/- CsA in a scratch-wound healing assay followed in an IncuCyte. B76 cells were seeded in 96-well plates (Essen image lock) and scratch wounds made simultaneously in all wells using the wound maker tool. Relative wound density (RWD), defined as the ratio of the cell density in the wound over the cell density outside the wound, was measured every second hour for up to 22 h (A). Open markers are for control (diamond) and IT 10 ng/ml (triangle) and filled markers are for CsA (diamond) and the combination of CsA and IT 10 ng/ml (triangle) or IT 1 ng/ml (circle). Average RWD after 22 h in five independent experiments is summarized in (B). The value for the control wells in each experiment is taken as 100% and used for normalization. Statistical significance was calculated using the T-test (2-tails, unequal variance).