H & E staining and observation of sheep OSE cell scrapings prior to immunomagnetic separation. OSE cell smear obtained after scraping sheep ovaries observed under (A) inverted microscope and (B, C) after H & E staining. As evident the OSE smear comprises of epithelial cells (arrow), small sized VSELs (dotted circle), OGSCs (asterisk) germ cell nests (GCN) and red blood cells (RBCs). H & E staining shows the presence of epithelial cells with pale stained nucleus, abundant cytoplasm and pink colored RBCs which were devoid of nuclei. Stem cells of two distinct sizes with dark stained nuclei and minimal cytoplasm including VSELs and OGSCs were observed along with small clusters which were the germ cell nests. Scale bar = 20 μm.