Figure 6

Live-cell imaging of cytoplasmic streaming and mitochondrial cloud (MitoTracker staining and Cytochrome c localization) in somatic fibroblasts and oocyte-like structures. The somatic mesenchymal fibroblast cells (top panel) show subtle change in morphology (arrowhead) due to the dynamic cytoplasmic streaming movement or blebbing typically observed at cell periphery during its development in culture at different time intervals at (A) 1 second (B) 5 seconds (C) 20 seconds and (D) 30 seconds from initiation of observation. The central region of small oocyte-like structure (middle panel) shows concentration of electron dense material (white asterisk). Note the gradual change in shape near its periphery (arrowhead) due to the subtle cytoplasmic movement imaged at (E) 1 second (F) 15 seconds (G) 30 seconds. Dense ring of mitochondrial cloud (lower panel) was visible within the oocyte-like structure in live cultures by (H) MitoTracker green FM staining and (I) immuno-localization of Cytochrome c (J) Mouse negative control was maintained by omission of primary antibody. Scale bar = 20 μm in A-G and 5 μm in H-J.