CD44 and CD168 expression in ovarian cancer cell lines. (A) The CD44 expression was measured by flow cytometry and analyzed with Cell Quest Pro software in ovarian cancer cell lines OVCAR-3, IGROV-1 and SKOV-3 as well as in REH lymphoblasts (negative control) and HeLa cells (positive controls). The auto fluorescence level was determined with an isotype control antibody (empty area). The CD44 expression was determined with an anti-CD44 antibody (full area) leading to the determination of the percentage of CD44 positive cells (M1). (B) The CD168 level was determined by western blot using a specific antibody in ovarian cancer cell lines OVCAR-3, IGROV-1 and SKOV-3 as well as in REH lymphoblasts and HeLa (positive controls). Immunocomplexes were visualized with a photon camera.