Effects of HA-CMC barrier on ovarian cancer cell line proliferation in vitro . (A, B, C) Ovarian cancer cell lines OVCAR-3 (A), IGROV-1 (B) and SKOV-3 (C) were cultured in the presence of control medium (RPMI), 1 cm2 of control absorbable material, or 1 cm2 of HA-CMC barrier. After 1, 2, 4 and 6 days of culture, cell number was counted with a hemocytometer. (Mean number of cells +/− SEM, n = 3). (D) Doubling times were calculated from previous proliferation curves according to the following formula: doubling time (hours) = t x [ln (2) / [ln (C1) – ln (C0)]] with t corresponding to the duration of the exponential phase, C0 to the initial cell concentration and C1 to the final cell concentration (between the beginning and the end of the exponential phase). (Mean doubling time +/− SEM, n = 3).