Figure 1

Tribbles genes expression in murine and bovine cumulus cells (CC) surrounding immature and mature oocytes in vivo . CC were recovered from either immature oocytes originating from small follicles (immature) or from metaphase-II oocytes from in vivo mature cumulus-oocyte complexes (COCs). A. Data from murine and bovine microarray analyses [11]. Histograms present mean expression values of six independent samples as detected by microarray hybridizations with correspondent p-values calculated using Fisher’s exact test and Benjamin-Hochberg correction). B. RT-qPCR analysis of Tribbles gene expression in CC. Graphs present mean +/−SEM of normalized expression values of five independent replicates. For statistics Mann–Whitney test was performed. Asterisks indicate significant differences (*p < 0.05, **p < 0.01; ***p < 0.001).