Expression of Tribbles family members in bovine cumulus cells and oocytes in relation with meiotic progression in vitro. A. RT-qPCR detection of TRIB1, TRIB2 and TRIB3 transcripts in bovine CC before (CC0) and after in vitro culture (CCIVM) and in immature oocytes (IO). Amplicon size is indicated on the left in base pairs (bp). B. Kinetics of meiotic progression in bovine oocyte during 24 h IVM as described [34, 61]. GV – germinal vesicle, GVBD – GV breakdown; Meta-I –metaphase-I, Telo-I – telophase-I, Meta-II – metaphase-II. C. Kinetics of TRIB1, TRIB2 and TRIB3 gene expression in bovine CC during IVM. RT-qPCR was performed on CC recovered from the COCs at 0 h, 6 h, 18 h and 24 h of IVM. Graphs present means +/−SEM of normalized expression values of 4 independent CC samples. Different letters indicate a significant difference (ANOVA and Tukey’s post-hoc test, p < 0.05). D. Detection of TRIB1, TRIB2 and TRIB3 proteins in bovine COCs. Western blots using anti-Tribbles antibodies were performed on COCs collected at 0 h, 4 h, 6 h 18 h and 24 h IVM; TUBA was used as a loading control.