Intracellular localization of TRIB1, TRIB2 and TRIB3 proteins in bovine cumulus cell cultured in vitro . Cells were fixed after 60 h of culture and double immunofluorescence was performed using primary antibodies against TUBA (red) and either rh TRIB1, or TRIB2 or TRIB3 proteins (green). For the negative control, primary antibody was replaced by mice and rabbit IgG. Chromatin is labeled blue. Nucleus (n), Metaphase (Meta), anaphase (Ana) and telophase (Telo) are indicated in some of the cells.