Figure 4

Growth inhibition and lethality of antiprogestins towards IGROV-1 and IGROV-1 PTES cells. Cells were plated in equal numbers, allowed to attach to the surface of the culture plates for 24 h, and then were treated with either vehicle (DMSO) or the indicated concentrations of each one of the antiprogestins for 96 h. (A) Total number of cells was recorded at the beginning of the experiment and after 96 h of treatment. The growth of the treated groups was expressed as percentage of control. (B) Viability was assessed with the Guava ViaCount application in a similar experimental approach to that shown in the left panel when assessing cell growth. (C) Following a similar treatment protocol as above cells were collected, fixed in 4% paraformaldehyde, stained with propidium iodide, and analyzed by cytometry using the Guava cell cycle application. Bars, mean ± s.e.m. (n = 3).