Figure 2
Comparison of ovarian area, follicle numbers and follicles at each stage. (A) Mean ovarian volume (mm2) in DHEA-treated vs. untreated ovaries. The height, major axis and minor axis of each ovary were measured in 0.01 mm increments; ovarian volumes were then calculated using the formula, Volume = (4 π a b c) / 3, where a = height, b = length of the major axis, and c = length of the minor axis. Values are medians ± 95% confidence intervals of the median. Average ovarian volumes were significantly smaller than control in the day-30 DHEA group. (B) Total number follicle numbers were significantly higher than control in the day-15 DHEA group. (C) Percentages of primordial follicles were significantly higher than control in the day-15 and day-30 DHEA groups. (D) Percentages of primary follicles were significantly higher than control in the day-30 DHEA group. E) Percentages of intermediary follicles (early preantral, late preantral and early antral) were significantly lower than control in the day-30 DHEA group. (F) Percentages of atretic follicles were significantly higher than control in the day-30 DHEA group. *P < 0.05; **P < 0.01.