Comparison of ovarian AMH immunostaining in DHEA-treated and control rats. AMH was detected immunohistochemically and scored as described in the Methods. (A) AMH staining in the day-7 DHEA and control groups. (B) AMH staining in the day-15 DHEA and control groups. (C) AMH staining in the day-30 DHEA and control groups. (D) Percentages of primordial follicles expressing AMH at the indicated levels. Staining intensity was significantly greater than control in the day-15 and day-30 DHEA groups. *P < 0.05; **P < 0.01.