Centrosome amplification in ovarian cancer. Ovarian cancer and normal adjacent tissue were stained with α-tubulin (red), γ-tubulin (green), and DAPI (blue) to visualize microtubules, centrosomes, and DNA, respectively. A. Confocal micrographs representing centrosome amplification status in ovarian normal, low-grade cancer and high-grade cancer tissue. B. Bar graph representation of percent cells showing centrosome amplification in ovarian and normal adjacent tissue. 500 cells were counted in each sample. C. Confocal microscopic images represent cells throughout the sequential stages of the cell cycle. Arrows indicate presence of centrosome clustering. Scale bar, 5 μm. D, E. Bar graph representation of percent cells showing multipolar mitosis and centrosome clustering in ovarian and normal adjacent tissue. 500 cells were counted in each sample. p < 0.05.