Table 1 Primers, fragment length and enzymes used for RFLP analysis
Gene/SNP | Primers (5′-3′) | Fragment lengths (bp) | Enzyme | RefSNP |
|---|---|---|---|---|
VEGFR2- 604 | Fw: CAAACTTTCACTAGGGCTCTTCGT | 290;174;116 | Bsm I | 2071559 |
Rev: AGCCACAAGGGAGAAGCGGATA | ||||
VEGFR2-1192 | Fw: TGAGGTTAAAAGTTCTGGTGTCCCTGTT | 262;232;30 | BstZ17 I | 2305948 |
Rev: AATGTACAATCCTTGGTCACTCCGGGGTA | ||||
VEGFR2-1719 | Fw: CCTCCTGTATCCTGAATGAATCT | 404;191;213 | Alu I | 1870377 |
Rev: GCCTCACATATTATTGTACCATCC | ||||
VEGF-405 | Fw: ATTTATTTTTGCTTGCCATT | 304;191;213 | BsmF 1 | 2010963 |
Rev: GTCTGTCTGTCTGTCCGTCA | ||||
VEGFR1-519 | Fw: GTGGCAACTTTGGGTTACCCA | 665;520;145 | Nsp I | 111458691 |
Rev: CCTGACCCCTTCAGACTGTCC | ||||