Figure 3

GNAI2 expression correlates with tumor stage. A. Data derived from GDS3297. IIIB n = 9, IIIC n = 23. Nonparametric p < 0.04 B. Data derived from TCGA, Nature 2011. Normal n = 8, IIA n = 2, IIB n = 4, IIC n = 8, IIIA n = 4, IIIB n = 14, IIIC n = 230, IV n = 53. Kruskal-Wallis p < 0.02. Normal versus IIIA nonparametric p < 0.009, IIIB Nonparametric p < 0.006, IIIC Nonparametric p < 0.004, IV Nonparametric p < 0.007. C. Data derived from GSE6008: stage III n = 2, IIIC n = 25, IV n = 5. Kruskal-Wallis ANOVA p < 0.03, Gaussian ANOVA p < 0.05, Gaussian III vs. IV p < 0.04, Gaussian IIIC vs. IV p < 0.05. Anderson-Darling Normality for IIIC p > 0.6 and for IV p > 0.9. Stage IIIC and stage IV were also statistically different using a parametric T-test. Expression is presented as HPRT1 normalized ∆∆CT. D. Methylation of GNAI2 gene correlates inversely with GNAI2 message expression. Methylation values reported as beta values.