Figure 2

EpoR is expressed by murine and human VSELs. Panel A. RQ-PCR results for EpoR expression by purified murine (left panel) and human (right panel) VSELs, HSCs, and MNCs. Combined data from three independent experiments are shown. Panel B. Immunostaining of EpoR and Oct4 protein in purified murine VSELs (left panel) and HSCs (right panel). The images were acquired using an Olympus FV1000 confocal microscope. A representative staining is shown. The experiment was performed on three independent cell sorts. Panel C. Detection of EpoR expression by FACS among hCB-derived VSELs. The left panel presents the percentages of CD34⁺ and CD133⁺ VSELs that express EpoR. The level of expression of EpoR on UCB-derived VSELs is ~20% for the population of Lin^-/CD45^-/CD34⁺ VSELs and ~15% for the population of Lin^-/CD45^-/CD133⁺ VSELs. The right panel shows one representative set of results out of ten FACS analyses.