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Figure 3 | Journal of Ovarian Research

Figure 3

From: Positive effects of prolonged caloric restriction on the population of very small embryonic-like stem cells – hematopoietic and ovarian implications

Figure 3

Effect of CR on morphology of ovaries and testes. Panel A. Ovaries of 10-month-old C57B1/6 mice fed AL (a, c, and e) or on CR (b, d, and f). a and b. In both groups, primary (PF), preantral (PAF), and antral (AF) follicles and corpus luteum (CL) are visible. We also observed primordial follicles and a greater number of atretic follicles (red arrowheads) in the CR group (b) than in the AL-fed group (a). Hematoxylin + eosin staining. Magnification: x100. Inset in panel b represents primordial follicles in an ovary from a mouse on CR. Magnification, x400. c-f. Immunohistochemical reaction for growth hormone receptor (GHR) expression in ovaries of mice fed AL (c and e) or on CR (d and f). High expression of GHR in ad libidum(AL)-fed mice was noted in granulosa cells (red arrow), preantral and antral follicles and zona pellucida (asterisk), ovarian surface epithelium (black arrowhead), and blood vessel endothelium (BV, c and e). By contrast, expression of GHR in CR mice was found only in a few granulosa cells (red arrow) of Graffian follicles (GF), and no expression of GHR was observed in granulosa cells of preantral (PAF), primary (PF), and primordial (PRF) follicles (d and f). Magnification: panels c and d, x100; panels e and f, x200. Representative images are shown. Panel B. The number of follicles at different stages of development in ovaries of 10 month-old female C57B/6 mice on CR compared with controls fed AL. The higher number of primordial, primary, preantral and atretic follicles in ovaries of mice on CR than in ovaries of mice fed ad libidum. P < 0.01. Panel C. Testes of 10-month-old C57B1/6 mice fed AL (panels a and c) or on CR (panels b and d). Magnification: panels a and b, x100; panels c and d, x200. PAS staining. Representative images are shown.

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