Figure 3
Inhibition of ovarian cancer growth in mice treated with hUCMSCs-LV-IL-21. A. The pictures represent the ovarian cancer bearing mice at five weeks after challenge of 5 × 106 SKOV3 cells along with injection of various hUCMSCs; B. The tumor sizes were analyzed and indicated the 301.03 ± 23.78 mm3, 219.80 ± 15.67 mm3, 200.96 ± 19.97 mm3 and 94.20 ± 15.57 mm3 in the mice treated with PBS control, the hUCMSCs, hUCMSCs-LV-Vec and in the hUCMSCs-LV-IL-21 in order; C-E. Levels of IL-21, IFN-γ and TNF-α in murine serum were detected by ELISA assay, respectively; F. IL-21 expression was shown in the tumor tissues from the mice treated with hUCMSCs-LV-IL-21. However, no specific IL-21 band was shown in the tumor tissues from the mice treated with hUCMSCs-LV-Vec, hUCMSCs and PBS, respectively; G-H. The mRNA expression of NKG2D and MIC A detected by the real time RT-PCR in tumor tissues from the mice treated with hUCMSCs-LV-IL-21, hUCMSCs-LV-Vec, hUCMSCs and PBS, respectively. *p < 0.05, **p < 0.01, and ***p < 0.005. ns: no statistical significant.