Figure 1

Knock-down efficiency of the AQP5 shRNA constructs. (A) Representative images of 3AO cells transfected with indicated plasmids; scale bar, 50 μm. Levels of AQP5 mRNA (B) and protein (C) in 3AO cells transfected with indicated shRNA contrasts. (D) Representative images of 3AO cells infected with indicated lentivirus; scale bar, 50 μm. Expression levels of AQP5 of 3AO cells treated with indicated virus determined by qPCR (E) and western blotting (F).