Relative mRNA abundance in granulosa cells of the largest follicle after intrafollicular injection of fulvestrant. A new follicular wave was induced (detailed in Methods) and 100 μM fulvestrant or saline was intrafollicularly injected when the largest follicle reached a diameter between 7 to 8 mm. Granulosa cells were recovered from saline (n = 3) and fulvestrant (n = 3) treated follicles at 12 h after intrafollicular injection. Abundance of CYP19A1, LHCGR, ESR1 and ESR2 genes are expressed as mean ± SEM. * indicates statistical difference (P ≤0.05) between groups.