Figure 3
Western blot analyses to detect AQP5 in cancerous ovaries and ascites-derived chicken ovarian cancer (COVCAR) cells. Protein extracts from ovary tissue and COVCAR cells were subjected to Western blot analysis under reducing conditions. AQP5 protein was detected by immunostaining using anti-human AQP5 antibody (–Blocking Peptide) and chemiluminescence. Specificity of AQP5 antibody was determined by preadsorption of anti-human AQP5 antibody with human AQP5 peptide (+Blocking peptide). CC-COVCAR cell lysate, CO-cancerous ovary lysate, M- molecular weight.