Overexpression of KLK6 and KLK7 genes was observes in OVC cell lines. (A) Established OVC cell lines representing different ages, stages and subtypes were selected and tested by end-point PCR for expression of known OVC genes (CA-125, HE4, and CEA) relative to normal ovarian epithelial cell lines. Amplified cDNAs were qualitatively compared following electrophoretic separation as ethidium bromide-stained bands on agarose gels. (B) Gene expression of KLK6 and KLK7 in OVC cell lines (solid black bars) and normal ovary cell lines (N) was analyzed by qRT-PCR and normalized against a “primary-like” normal ovarian cell line (IOSE523; solid gray bar). IOSE523 cells begin to senesce after twenty passages while FIOSE118 cells are immortal. The mean fold change represents triplicate measurements, and standard error bars are shown.