Figure 1
From: Succinate dehydrogenase subunit B inhibits the AMPK-HIF-1α pathway in human ovarian cancer in vitro
SDHB silencing in SKOV3 and A2780 cells. SKOV3 and A2780 cells were treated with siSucA or siSucC oligonucleotides, (A) real-time PCR (24 h) and (B) western blot (48 h) were performed to detect SDHB mRNA and protein levels, respectively. 48 h after siRNA transfection, SDH activity (C) and ATP level (D) were examined, p-AMPKα, AMPKα (E) and p-P38, P38 (F) were measured in SKOV3 and A2780 cells. Mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001.